Our contribution to the community

Information on how to order seeds from a tobacco diversity panel presented in our recent manuscript.

• In a recent manuscript bioRxiv, we assembled the allotetraploid nicotiana tabacum, genotype and phenotyped an entire GenBank. Results from our study will likely accelerate aspects of tobacco research that were previously hindered by the complexity of the polyploid genome and a lack of publicity for research driven by the private sector, with benefits in plant functional genomics, crop improvement research, and the production of biopharmaceutical compounds and biofuel. To make this resource available to the community, we selected 310 accessions covering the majority of the genetic and phenotypic diversity, and have made the seeds of these accessions available for ordering. Our intention is for this collection to remain actively curated as ever more genomic data are produced and a wide range of phenotypic data are generated.

General information

• The list of accession, their genotype and phenotype are available from supplements of recent our manuscript.
• The seeds were packed in plastic bags one accession per bag, and each bag contains roughly 100 seeds.
• Each bag comes with a cost of 25 Euro to cover operational cost at our side.
• A packed order for 310 accession is 5000 Euro.
• Cost of Postage is not covered.
• For research only, commericial use is strictily prohibited.

Ordering information:

Please drop an email to zanyanjun@caas.cn with the following information :

1. Your mailing address
2. An import permit for plant seeds from your country
3. A short description of how you will use the seeds

Normally, we will resopnd to your request within 10 working days with detailed instruction on how to proceed.

Wet-lab Protocal

• Zan Y et al. A high throughput, cost-efficient library preparation protocol for large-scale next generation sequencing. protocols.io. 2018. DOI = dx.doi.org/10.17504/protocols.io.rt8d6rw.

Software packages and pipline

• BEFDR: A R package for multi-locus association analysis using a backward elimination
  approach. Available in Github
• A Python Snakemake pipeline for low coverage genotype imputation (R/Python/Perl). Available in Github
• TD: A R package for downstream pipeline for handling low coverage imputation. Available in Github

Teaching materials

• A full course in GWAS, GS and multi-omics analysis Link to video
• A full tutorial on GWAS in R GenABEL Link to video

Conferernece talks in quantattive genetics

• Epistasis, genotype by environment intearction and across envrionment complex trait varaition Link to video
• 60-year artificial bi-directional selection and efforts from 3 generation of distinguished scientist Link to video